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本研究对我国一类检疫性有害生物菜豆萎蔫病菌Ｃｕｒｔｏｂａｃｔｅｒｉｕｍｆｌａｃｃｕｍｆａ－ｃｉｅｎｓｐｖ．ｆｌａｃｕｍｆａｃｉｅｎｓ（Ｃｆ）进行种子检测鉴定技术的研究，发现５２３，ＮＢＹ＋ＴＴＣ和ＮＢＹ＋刚果红三种培养基均可作为分离培养基；对带菌种子分离的灵敏度为１．８×１０２ｃｆｕ／ｍｌ，病叶柄和茎是分离的最佳部位，病菌可通过种子传给种苗引起种苗发病，发病时间为７～２０天，针刺接种是Ｃｆ最有效的接种方法，接种发病率为１００％。使用标准化的Ｂｉｏｌｏｇ鉴定系统鉴定病原，在种水平的鉴定准确率达９８．５％。
In this study, Curtobacterium flavocfa-cienspv, a quarantine pest in China, flacumfaciens (Cf). The results showed that 523, NBY + TTC and NBY + Congo red could all be used as the isolation medium. The sensitivity of the fungi to the isolation of the seed was 1.8 × 102 cfu / ml, Is the best part of the separation, germs can be caused by seed seedlings caused by disease, the onset time is 7 to 20 days, acupuncture is the most effective Cf inoculation method, the incidence of vaccination was 100%. The identification of pathogenic bacteria using a standardized Biolog system identified 98.5% accuracy at the species level.