目的：研究山鉼药材体积分数70％乙醇提取物中总黄酮与二苯乙烯类化合物的分离工艺。方法：山鉼原药材提取挥发油后，用体积分数70％的乙醇超声提取，提取物用不同浓度的乙醇梯度洗脱，收集洗脱液，参照山鉼指纹图谱，用高效液相色谱法测定其中所含的总黄酮及二苯乙烯类化合物的量。结果：山鉼药材体积分数70％的乙醇提取物用体积分数65％乙醇洗脱至1500 mL 时二苯乙烯类化合物的含量最高且总黄酮的量较少；用体积分数90％的乙醇洗脱至2500 mL 时总黄酮的量最高。结论：确立了山鉼药材体积分数70％乙醇提物中二苯乙烯类及总黄酮的分离工艺，为以后山鉼有效成分的提取分离及进一步研究奠定了基础。“,”Objective:Study the separation process of total flavonoids and toluylene compounds from ethanol extract of Lindera reflexa. Methods:Volatile oil were extracted from Lindera reflexa Hemsl raw material,with volume fraction of 70% ethanol ultrasonic extraction, extract with different concentration ethanol gradient elution and collecting the eluent,the reference fingerprint atlas of Lindera reflexa, determination of the contents contained flavonoids and stilbenes compounds by HPLC.Results:70% ethanol extract of Lindera reflexa used the volume fraction of 65% ethanol elution to 1 500 mL of stilbene compounds content highest and total flavonoids of less;with 90% ethanol elution to 2 500 mL when the highest amount of total flavonoids.Conclusion:Establish separation process of total fla-vonoids and toluylene compounds from ethanol extract of Lindera reflexa and lay a foundation for the extraction and separation of the ef-fective components in Lindera reflexa.