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目的为了进一步探讨ＨＣＣ中ｐ５３基因密码子２４９突变的作用机理。方法收集密码子２４９突变率为３２９％的７０例南方地区手术切除ＨＣＣ标本，采用ＳＳＣＰ、ＩＨＣ和ＲＮＡ斑点杂交分析方法，深入研究了密码子２４９突变伴ＬＯＨ情况及其对ｐ５３基因转录和翻译的影响。结果９０％的密码子２４９突变伴ＬＯＨ。突变组中，ｐ５３蛋白和ｐ５３ｍＲＮＡ检出率分别为９１３％和９５７％，两者均显著高于无突变组（Ｐ＜０００１）。相关分析显示，ｐ５３基因的转录与翻译之间高度相关（ｒ＝０．８２０８）。结论ＬＯＨ是使ｐ５３基因密码子２４９突变发挥致癌作用的主要因素。ｐ５３基因密码子２４９的突变导致了ｐ５３基因在转录和翻译两水平上的增加，其中转录增加是ｐ５３蛋白表达增加的重要原因之一
Objective To further explore the mechanism of mutation of p53 gene codon 249 in HCC. Methods 70 cases of HCC specimens with a codon 249 mutation rate of 32.9% were collected and used SSCP, IHC and RNA dot blot analysis methods to deeply study the situation of codon 249 mutation with LOH and transcription of p53 gene. The impact of translation. Results 90% of codon 249 mutations were associated with LOH. In the mutant group, the detection rates of p53 protein and p53 mRNA were 91. 3% and 95. 7%, respectively, both of which were significantly higher than those of the non-mutation group (P <0.001). Correlation analysis revealed a high correlation between transcription and translation of the p53 gene (r=0.8208). Conclusions LOH is the major factor causing the mutation of p53 gene codon 249 to play a carcinogenic role. Mutations in the p53 gene codon 249 result in increased transcription and translation of the p53 gene, and increased transcription is one of the important causes of the increased p53 protein expression.