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目的探讨颈交感神经干离断(TCST)对应激大鼠胃粘膜损伤的保护作用。方法雄性 SD大鼠30只,随机分为3组(n=10):假手术组(A组)、假手术+浸水组(B组)和TCST+浸水组(C 组)。A、B组暴露并分离右颈交感神经干,不离断;C组在距颈上神经节下3mm处离断颈交感神经干并将断端结扎。B、C组大鼠垂直浸水6b取出,测定胃粘膜血流量(GMBF)、血浆内皮素-1(ET-1)及血清一氧化氮(NO)浓度,肉眼下按Guth标准评价胃粘膜损伤程度,计算粘膜损伤指数(UI),光镜下观察胃粘膜病理性改变。结果与A组比较,B、C组GMBF降低,血浆ET-1浓度升高,B组血浆NO浓度升高(P<0．01);与B组比较,C组GMBF升高,UI及血ET-1、NO浓度降低(P<0．01)。A组胃粘膜无明显损伤,C组粘膜损伤程度较B组减轻。结论 TCST对浸水应激大鼠胃粘膜损伤有保护作用,与降低血中ET、NO水平,增加GMBF有关。
Objective To investigate the protective effect of cervical sympathetic trunk (TCST) on gastric mucosal lesion in stress rats. Methods Thirty male Sprague Dawley rats were randomly divided into three groups (n = 10): sham operation group (A), sham operation + water immersion group (B) and TCST + water immersion group (C). Groups A and B exposed and isolated the right cervical sympathetic trunk without disconnection. In group C, the cervical sympathetic trunk was separated 3 mm below the superior cervical ganglion and ligatured. The rats in groups B and C were immersed in water for 6 hours, and the gastric mucosal blood flow (GMBF), plasma endothelin-1 (ET-1) and serum nitric oxide (NO) The mucosal damage index (UI) was calculated and pathological changes of gastric mucosa were observed under light microscope. Results Compared with group A, GMBF decreased in group B and C, plasma ET-1 concentration increased and plasma NO concentration increased in group B (P <0.01). Compared with group B, group C increased GMBF, UI and blood ET-1, NO concentration decreased (P <0.01). A group of gastric mucosa no obvious damage, C group mucosal lesion lesser than B group. Conclusion TCST has a protective effect on gastric mucosal injury induced by flooding in rats, which is related to the decrease of ET and NO in blood and the increase of GMBF.