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Object. The effects of ATP-introduced a rise in cytosolic free Ca2+ concentration and inhibition of nitric oxide were investigated. Method. Measurement of free Ca2+([Ca2+] i)of cultured rat tail arterial smooth muscle cells using Fura-2/AM dual excitation wavelength spectrofluorometer. Results. There are two components of [Ca2+] i can be evoked by ATP. One part is Ca2+ entry from Ca2+ channel and formed a plateau. The another part is a peak that released from Ca2+ store. Both of them can be inhibited by NO. Conclusion. The ATP induced [Ca2+] i rise that release Ca2+ from both Insp 3 and ryanochine receptors and Ca2+ entry through calcium channels. The inhibition of NO on ATP induced [Ca2+] i rise that was mediated by cGMP.
Object. The effects of ATP-introduced a rise in cytosolic free Ca2 + concentration and inhibition of nitric oxide were investigated. Method. Measurement of free Ca2 + ([Ca2 +] i) of cultured rat tail arterial smooth muscle cells using Fura-2 / AM dual There are two components of [Ca2 +] i can be evoked by ATP. One part is Ca2 + entry from Ca2 + channel and formed a plateau. The another part is a peak that released from Ca2 + store. Both of them can be inhibited by NO. Conclusion. The ATP induced [Ca2 +] i rise that release Ca2 + from both Insp 3 and ryanochine receptors and Ca2 + entry through calcium channels. The inhibition of NO on ATP induced [Ca 2+] i rise that was mediated by cGMP.