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对营养竞争拮抗测定方法进行了改进,对接法将芽孢杆菌JPC-2与小麦纹枯病菌接种于大豆麦麸(SWB)培养基上,JPC-2可在SWB培养基上迅速扩展并完全抑制小麦纹枯病菌的菌丝生长。田间试验表明,芽孢杆菌JPC-2液体菌剂(107cfu/mL,1∶100)处理种子对小麦纹枯病的冬前期防效高于扬花期防效,防治效果为61.9%,高于3%苯醚甲环唑包衣处理(用量为种子质量的0.3%);对小麦根腐病的防治效果可达46.8%,与空白对照差异显著(p<0.05);并使小麦增产73.5%,高于3%苯醚甲环唑种衣剂。芽孢杆菌JPC-2固体菌剂(106cfu/mL,225kg/hm2)沟施于土壤,再播种液体菌剂包衣(107cfu/mL,药种比1∶30)的小麦种子,对小麦纹枯病菌的冬前期防效为68.9%,扬花期防效为59.7%,小麦增产38.9%,与0.2%戊唑醇种衣剂(1∶50)增产效果相当。
The method of antagonistic assay of nutrition competition was improved. The inoculation method was inoculated on the medium of soybean bran (SWB) with Bacillus JPC-2 and Rhizoctonia cerealis. JPC-2 could rapidly expand on SWB medium and completely inhibit Mycelial growth of wheat sheath blight. Field experiments showed that the control effect of the seed of Bacillus JPC-2 liquid bacterium (107cfu / mL, 1: 100) on wheat sheath blight was higher than that of flowering stage, the control effect was 61.9%, higher than 3% Difenoconazole, and difenoconazole (0.3% of seed weight); the control effect on root rot was up to 46.8%, which was significantly different from the control (p <0.05); the yield of wheat was increased by 73.5% 3% difenoconazole seed coating agent. Bacillus thuringiensis JPC-2 solid fungi (106cfu / mL, 225kg / hm2) were dug in the soil, and wheat seeds of liquid bactericide coating (107cfu / mL, The pre-winter control of fungi was 68.9%, the flowering-stage control effect was 59.7%, the yield of wheat increased by 38.9%, and the effect was similar to that of 0.2% tebuconazole seed coating agent (1:50).