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本文报道对清洁级实验动物应排除的四种病毒（淋巴细胞脉络丛脑膜炎病毒、小鼠脱脚病病毒、鼠肝炎病毒和仙台病毒）抗体玻片酶免疫（ＥＩＡ）检测试剂盒的研制。四种病毒感染的细胞和对照细胞经冷丙酮固定于载玻片上制成特异性抗原和对照抗原，此四种病毒的抗血清各１０份和ＳＰＦ小鼠血清２０份分别与四种病毒的特异性抗原和对照抗原进行ＥＩＡ交叉试验，结果显示，抗原只与其相应抗血清发生特异性显色反应，与非特异性小鼠血清和ＳＰＦ小鼠血清不显色。与ＨＩ或ＥＬＩＳＡ方法比较，通过对１１２份普通小鼠血清进行测试，结果表明，ＥＩＡ对仙台病毒抗体的检出率（１９．６％）显著高于（＜０．００５）ＨＩ（６．３％），对小鼠脱脚病病毒抗体的检出率（２３．３％）与ＨＩ（２１．４％）无显著性差异（Ｐ＞０．０５）。ＥＩＡ对淋巴细胞脉络丛脑膜炎病毒和鼠肝炎病毒抗体的检出率分别为１．８％和７１．２％，ＥＬＩＳＡ对两种病毒抗体的检出率分别为１．８％和６７．６％，两种方法对两种病毒抗体的检出率无显著性差异（Ｐ＞０．０５）。重复性试验表明两批四种病毒抗体试剂盒对１０８份小鼠血清两次测定的符合率为９６～１００％。四种病毒的ＥＩＡ抗原在－１８℃保存１２个月或在２－８℃保存３
This article reports the development of an antibody immunohistochemistry (EIA) kit for the detection of four viruses (lymphocytic choriomeningitis virus, mouse ankle virus, mouse hepatitis virus and Sendai virus) that should be excluded from clean-grade laboratory animals. The four virus-infected cells and control cells were fixed on glass slides with cold acetone to make specific antigens and control antigens. The antiserum of each of the four viruses and the serum of SPF mice were respectively distinguished from the four viruses EIA crossover test between the antigen and the control antigen showed that the antigen only had a specific color reaction with its corresponding antiserum and did not develop color with nonspecific mouse serum and SPF mouse serum. Compared with HI or ELISA methods, the results showed that the detection rate of EIA antibody (19.6%) was significantly higher than (<0.005) HI (6.3 %). There was no significant difference (23.3%) between the antibody and the HI (21.4%) in the mice (P> 0.05). The detection rates of EIA against lymphocytic choriomeningitis virus and murine hepatitis virus were 1.8% and 71.2%, respectively, and the detection rates of ELISA against the two virus antibodies were 1.8% and 67.6%, respectively %. There was no significant difference in the detection rate of the two antibodies between the two methods (P> 0.05). Repeatability tests showed that the coincidence rates of two batches of four kinds of virus antibody kit to two determinations of 108 mice serum were 96-100%. The EIA antigens of the four viruses are either stored for 12 months at -18 ° C or 3 for storage at 2-8 ° C