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为探讨生理性深层海水(PDSW)联合热疗对人胃癌SGC-7901细胞体外和体内的抗癌作用,通过在体外培养人胃癌SGC-7901细胞株,随机分为实验组、对照组和空白组,实验组加PDSW,对照组加生理盐水,空白组不做其他处理,分别在37℃、40℃和43℃热疗温度下培养。同时,将SGC-7901细胞注射至裸鼠皮下,建立移植瘤模型,随机分为5组,至肿瘤长至直径为1 cm时,分别进行H_2O+RT、H_2O+40℃、H_2O+43℃、PDSW+40℃、PDSW+43℃处理,然后绘制裸鼠肿瘤生长曲线,观察裸鼠肿瘤组织切片,并用实时荧光定量PCR检测细胞和组织中NF-κB基因表达情况,Western blotting检测NF-κB蛋白的表达情况。研究发现,与其他处理相比,PDSW联合热疗处理后肿瘤生长缓慢,肿瘤细胞的增长明显受抑,细胞和裸鼠皮下移植瘤组织中NF-κB基因和蛋白的表达量较低。说明PDSW联合热疗能抑制胃癌SGC-7901细胞中NF-κB基因和蛋白的表达,从而有效抑制胃癌细胞的增殖。
To investigate the anti-cancer effect of PDSW combined with hyperthermia on human gastric cancer SGC-7901 cells in vitro and in vivo, SGC-7901 cells were cultured in vitro and randomly divided into experimental group, control group and blank group , The experimental group plus PDSW, the control group plus saline, the blank group without any other treatment, respectively, at 37 ℃, 40 ℃ and 43 ℃ hyperthermia temperature culture. At the same time, SGC-7901 cells were injected subcutaneously into nude mice to establish xenograft model, which were randomly divided into 5 groups. When the tumor grew to 1 cm in diameter, H_2O + RT, H_2O + 40 ℃, H_2O + 43 ℃, PDSW + 40 ℃ and PDSW + 43 ℃. The tumor growth curve of nude mice was drawn. The tumor tissue sections of nude mice were observed. The expression of NF-κB gene in cells and tissues was detected by real-time fluorescence quantitative PCR. The expression of the situation. The study found that, compared with other treatments, PDSW combined with hyperthermia treatment slow tumor growth, tumor growth was significantly inhibited, cells and nude mice subcutaneously transplanted tumor tissue NF-κB gene and protein expression was low. PDSW combined with hyperthermia can inhibit the expression of NF-κB gene and protein in gastric cancer SGC-7901 cells, which can effectively inhibit the proliferation of gastric cancer cells.