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为了研究尼古丁对学习记忆的影响，我们利用免疫组织化学技术，研究尼古丁对跳台学习小鼠海马结构ｃ－ｆｏｓ蛋白表达的影响。结果显示，虽然５分钟的小鼠跳台学习成绩（错误数）实验组与对照组相比，差异没有显著性，但给药２小时后海马结构ｃ－ｆｏｓ蛋白表达有所不同，其中２ｍｇ／ｋｇ尼古丁剂量组在海马ＣＡ３区和齿状回部位的ｃ－ｆｏｓ阳性细胞数比１ｍｇ／ｋｇ尼古丁剂量组及对照生理盐水组显著增多（Ｐ＜０．０１），而１ｍｇ／ｋｇ尼古丁剂量组与对照生理盐水组差异无显著性。提示２ｍｇ／ｋｇ尼古丁剂量可能对学习记忆有损害作用。
In order to study the effect of nicotine on learning and memory, we used immunohistochemistry to study the effect of nicotine on c-fos protein expression in hippocampal formation in platform-learning mice. The results showed that there was no significant difference between the experimental group and the control group in the 5-minute jumping test scores (error number), but the expression of c-fos protein in the hippocampus was different after 2 hours of administration, of which 2 mg / kg The number of c-fos positive cells in hippocampal CA3 region and dentate gyrus in nicotine group was significantly higher than that in nicotine 1 mg / kg group and control group (P <0.01), while the dose of nicotine 1 mg / kg group was significantly higher than that in control group There was no significant difference in saline group. Tip 2mg / kg nicotine dose may have a detrimental effect on learning and memory.