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作者应用荧光显微镜、显微荧光光度计及扫描电镜方法,对不同周期型的21个种、株的丝虫微丝蚴体内有无发荧光的物质及微丝蚴的周期性进行了研究。荧光显微镜观察:所用的样本晾干,甲醇固定,不需染色,直接在射线波长为350～540微米的荧光显微镜下观察。结果:高度夜间周期性的正常株班氏丝虫、泰国马来丝虫及猫体的派特丝虫的微丝蚴均有弥漫性自身荧光及大量的荧光颗粒;低度夜间周期性的犬恶丝虫、南朝鲜马来丝虫、伦敦及东京株猫体的马来丝虫微丝蚴都有少量荧光颗粒;夜间前周期性的常型恶丝虫微丝蚴的荧光颗粒则更少;亚周期型波利尼西亚株的班氏丝虫,猫和狗体彭亨丝虫只在一些微丝蚴中检出少
The authors used fluorescence microscopy, micro-fluorophotometer and scanning electron microscopy methods to study the periodicity of the presence or absence of fluorescent substances and microfilariae in 21 species and strains of filamentous microfilariae of different cycle types. Fluorescence Microscopy: The samples used were air-dried and fixed in methanol without staining. They were observed directly under a fluorescence microscope with a wavelength of 350 to 540 microns. RESULTS: The microflora of filarial filariasis of Bancroftian thallus, thallus malayi and cat body of the normal strain highly nocturnal at night had diffuse autofluorescence and a large amount of fluorescent particles. The low nighttime periodic dog There is a small amount of fluorescent particles in the filariasis of the filariasis, the filariasis of the South Korea, the filariasis of the filariasis in London and Tokyo, and the less frequent fluorescent particles of the usual filarial microfilariae of night-time periodicity ; Bancroftian filariasis in Subperivical Polynesia strains, Paenoconian filariasis of cats and dogs were only detected in some microfilariae