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目的 :观察霉酚酸酯 (MMF)对炎症因子刺激下内皮细胞表面粘附分子ICAM 1表达的影响。 方法 :以TNFα(2 0 μg/L)刺激内皮细胞 ,细胞表面的ICAM 1蛋白表达以流式细胞术检测 ,内皮细胞ICAM 1mRNA表达采用逆转录 半定量PCR法进行测定。 结果 :TNFα(2 0 μg/L)刺激内皮细胞 2 4h ,内皮细胞表面的粘附分子ICAM 1的蛋白和mRNA表达明显上升。MMF可以抑制内皮细胞的ICAM 1蛋白和mRNA的表达 ,这一抑制作用随着MMF剂量的增加而增强。 结论 :MMF可以抑制内皮细胞粘附分子ICAM 1的表达
Objective: To observe the effect of mycophenolate mofetil (MMF) on the expression of adhesion molecule ICAM 1 on endothelial cells stimulated by inflammatory cytokines. Methods: Endothelial cells were stimulated with TNFα (20 μg / L). The expression of ICAM 1 on the cell surface was detected by flow cytometry. The expression of ICAM 1 mRNA in endothelial cells was determined by reverse transcription semi-quantitative PCR. RESULTS: After stimulated with TNFα (20 μg / L) for 24 hours, the expression of ICAM 1 protein and mRNA on endothelial cells increased significantly. MMF inhibited the expression of ICAM 1 protein and mRNA in endothelial cells, which was enhanced with the increase of MMF dose. Conclusion: MMF can inhibit the expression of endothelial cell adhesion molecule ICAM 1