论文部分内容阅读
目的研究一叶秋碱（ＳＥＣ）能否诱导Ｋ５６２细胞凋亡。方法细胞增殖抑制采用ＭＴＴ法；形态学研究采用电子和荧光显微镜；流式细胞仪和琼脂糖凝胶电泳检测ＤＮＡ断裂。结果ＳＥＣ１０～１６０ｍｇ·Ｌ－１呈剂量依赖性抑制Ｋ５６２细胞增殖（ｒ＝０９６１３，Ｐ＜００５）；ＳＥＣ作用４８ｈ后，电镜下可见细胞膜完整，核染色质边聚和凋亡小体；荧光显微镜下核染色质凝聚成点状结构；流式细胞仪出现凋亡峰；ＤＮＡ琼脂糖凝胶电泳可见“梯状”图谱。结论ＳＥＣ可诱导Ｋ５６２细胞调亡。
Objective To study whether YE can induce apoptosis of K562 cells. Methods MTT assay was used to inhibit cell proliferation; electron microscopy and fluorescence microscopy were used for morphological studies; DNA fragmentation was detected by flow cytometry and agarose gel electrophoresis. Results SEC 10～160mg·L-1 inhibited the proliferation of K562 cells in a dose-dependent manner (r=09613, P<005). After SEC treatment for 48h, the integrity of the cell membrane, the nuclear chromatin condensation and apoptosis were observed under electron microscope. The fluorescence microscopy showed that chromatin condenses into a punctate structure; flow cytometry shows apoptotic peaks; DNA agarose gel electrophoresis shows a “ladder” pattern. Conclusion SEC can induce apoptosis in K562 cells.