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目的:运用代谢组学方法研究积滞化热模型大鼠机体代谢表型的变化及清伙灵发挥干预作用的相关代谢途径。方法:实验分正常组、模型组和清伙灵组。模型组和清伙灵组给予特制饲料并ip阿托品(0.25 mg·kg-1),每日2次,连续造模7 d,从造模第4天开始,ig清伙灵(2.64 g·kg-1),每日1次。大鼠血清和尿液的代谢指纹利用核磁共振氢谱(1H-NMR)进行表征。综合使用主成分分析、正交偏最小二乘判别分析和单变量统计分析方法探讨积滞化热证引起的机体代谢表型的变化,鉴定及比较相关的代谢标志物。结果:与正常组比较,模型组的肛温、饮水量、饮食量等有明显改变(P<0.05)。在血清和尿液中分别筛选得到6种和7种与积滞化热证相关的代谢标志物。模型组大鼠机体代谢表型发生了明显的改变,脂质代谢、糖吸收和代谢、肠道菌群都受到不同程度的影响。清伙灵组与模型组比较差异有统计学意义(P<0.05)。结论:清伙灵能有效改善积滞化热证候大鼠的脂质和糖代谢失衡,并可调节肠道菌群。
OBJECTIVE: To study the changes of metabolic phenotypes in rats with stagnant heat and the related metabolic pathways by which Qinguoling exerts its intervention effect by means of metabonomics. Methods: The experiment was divided into normal group, model group and Qingliao group. The model group and Qingliaoling group were given special diet and ip atropine (0.25 mg · kg-1) twice daily for 7 days. From the fourth day after modeling, ig Qingluoling (2.64 g · kg -1) -1), once daily. Metabolic fingerprints of rat serum and urine were characterized by 1H nuclear magnetic resonance (1H-NMR). Principal component analysis, orthogonal partial least-squares discriminant analysis and univariate statistical analysis were used to explore the changes of metabolic phenotype caused by stagnant heat syndrome and to identify and compare the relevant metabolic markers. Results: Compared with the normal group, the rectal temperature, water intake, food intake and so on in the model group were significantly changed (P <0.05). Six kinds and seven kinds of metabolic markers related to stagnant heat syndrome were screened respectively in serum and urine. The metabolic phenotype of the model rats changed significantly, the lipid metabolism, carbohydrate absorption and metabolism, intestinal flora were affected to varying degrees. There was a significant difference between Qing-yu Ling group and model group (P <0.05). Conclusion: Qingluoling can effectively improve imbalance of lipid and glucose metabolism in rats with stagnant heat syndrome, and can regulate intestinal flora.