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目的:建立近似人类新生儿窒息的病理过程的新生鼠窒息心肌损伤模型,观察心肌细胞凋亡及使用三七总皂甙(TPNS)后心肌细胞凋亡的变化。方法:出生7天SD大鼠88只,雌雄不拘,随机分为对照组(n=8只),窒息组(n=40只),窒息+TPNS(n=40只)。将大鼠分别放入55ml的磨口瓶中,瓶内放入0.005kg的钠石灰,待大鼠安静后,窒息组,窒息+TPNS组塞紧瓶塞0.5h,取出吸氧120min后,放回母鼠身边继续喂养,分别于窒息后6h、24h、48h、72h、7天后处死;对照组放入55ml的磨口瓶中0.5h不塞瓶塞,取出吸氧120min后放回母鼠身边继续喂养,6h后全部处死。采用TUNEL法测定心肌细胞凋亡,每组各随机抽取一个标本做电镜片HE染色的光镜病理片。结果:①窒息组、窒息+TPNS组各时段的心肌病理切片有明显的心肌细胞坏死,炎性细胞浸润和出血;电镜切片下有心肌细胞线粒体普遍肿胀,细胞核肿胀,染色质边集,肌纤维融合,Z线不清,线粒体空泡,脊模糊稀疏;对照组光镜和电镜病理切片均正常;②窒息组,窒息+TPNS组各时段心肌细胞凋亡率明显高于对照组(P<0.01),有明显统计学差异;③窒息组各时段心肌细胞凋亡率明显高于窒息+TPNS组(P<0.05)。结论:窒息时心肌细胞凋亡率上升是心肌损伤的一种形式;使用三七总皂甙后能明显减轻心肌细胞凋亡率,提示三七总皂甙对窒息所致心肌损伤有明显保护作用。
OBJECTIVE: To establish a model of myocardial infarction in neonatal rat asphyxia, which is similar to the pathological process of asphyxia in human neonates, and observe the changes of cardiomyocyte apoptosis and cardiomyocyte apoptosis after using Panax Notoginseng Saponins (TPNS). Methods: Seventy - eight SD rats were randomly divided into control group (n = 8), asphyxia group (n = 40) and asphyxia + TPNS group (n = 40). The rats were placed in 55ml grinding mouth bottle, the bottle into the 0.005kg soda lime, until the rats were quiet, asphyxia group, asphyxia + TPNS group stuffed cork 0.5h, remove the oxygen after 120min Back to the side of the mother to continue feeding, respectively, 6h, 24h, 48h, 72h, 7 days after asphyxiation were sacrificed; control group into the 55ml mill mouth flask 0.5h plug stopper, remove the oxygen 120min after the mother back Continue to feed, all executed after 6h. Cardiomyocyte apoptosis was measured by TUNEL method. One specimen from each group was randomly selected for histopathological examination by HE staining. Results: ① Asphyxia group, asphyxia + TPNS group showed obvious myocardial necrosis, infiltration of inflammatory cells and hemorrhage in each pathological section of cardiomyocytes. Electron microscopy showed widespread swelling of mitochondria, nuclear swelling, chromatin margination and fusion of muscle fibers , Z line was unclear, mitochondrial vacuoles and spine were sparse and fuzzy. The light and electron microscope pathological sections of control group were normal. ② The apoptosis rate of myocardial cells in asphyxia group, asphyxia + TPNS group was significantly higher than that in control group (P <0.01) (P <0.05). (3) The apoptosis rate of myocardial cells in asphyxia group was significantly higher than that in asphyxiated + TPNS group (P <0.05). Conclusion: Asphyxia myocardial cell apoptosis rate is a form of myocardial injury; the use of notoginseng saponin can significantly reduce the rate of myocardial cell apoptosis, suggesting notoginseng saponins on myocardial injury caused by asphyxia have a significant protective effect.