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目的研究从萌芽到移栽4个不同的发芽阶段对丹参种苗生物量以及活性成分含量的影响。方法采集不同发芽阶段的丹参幼苗,测定其根长、芽长、根径及根重,利用HPLC测定丹参种苗根中脂溶性成分二氢丹参酮、隐丹参酮、丹参酮I和丹参酮IIA及水溶性成分迷迭香酸和丹酚酸B的含量。结果随着丹参种苗的萌发,除了根部的折干率下降了15.65%,丹参的各项生物学指标整体上呈现增长的趋势。根中脂溶性成分含量均呈现先增加后降低的趋势,当芽根值为0.25时,脂溶性成分含量达到最大,二氢丹参酮、隐丹参酮、丹参酮I和丹参酮IIA的含量分别为0.42 mg·g~(-1)、0.81mg·g~(-1)、0.59 mg·g~(-1)和4.67 mg·g~(-1)。水溶性成分含量呈现增长的趋势,当芽根值为0.28时,水溶性成分含量达到最大,迷迭香酸和丹酚酸B的含量分别为5.71 mg·g~(-1)和25.02 mg·g~(-1)。结论芽根值在0.25~0.28时,丹参中的脂溶性成分和水溶性成分达到或接近萌发阶段的最大值。在优选种苗时可以以芽根值作为选择的依据。
Objective To study the effects of four different germination stages from sprouting to transplanting on the biomass and the content of active ingredients of Salvia miltiorrhiza seedlings. Methods Salvia miltiorrhiza seedlings were collected at different stages of germination. The root length, shoot length, root diameter and root weight were measured. The lipid-soluble components such as DHT, Cryptotanshinone, Tanshinone I, Tanshinone IIA and water- Rosmarinic acid and salvianolic acid B content. Results With the germination of Salvia miltiorrhiza seedlings, all the biological indexes of Salvia miltiorrhiza showed an increasing trend except for the reduction rate of 15.65%. The content of fat-soluble components in root increased first and then decreased. When the root value was 0.25, the content of fat-soluble components reached the maximum. The content of dihydrotanshinone, cryptotanshinone, tanshinone I and tanshinone IIA were 0.42 mg · g ~ (-1), 0.81 mg · g -1, 0.59 mg · g -1 and 4.67 mg · g -1, respectively. The content of water-soluble components showed a trend of increasing. When the root value was 0.28, the content of water-soluble components reached the maximum, and the contents of rosmarinic acid and salvianolic acid B were 5.71 mg · g ~ (-1) and 25.02 mg · g ~ (-1). Conclusions When the shoot root value is between 0.25 and 0.28, the fat-soluble and water-soluble components in Salvia miltiorrhiza reached or approached the maximum of the germination stage. In the preferred seedling shoots can be taken as the basis for selection.