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目的探讨卵巢上皮性恶性肿瘤患者手术前后外周血Th1/Th2细胞因子的表达状况。方法选取2015年1月至2016年3月间复旦大学附属中山医院青浦分院收治的30例卵巢上皮性恶性肿瘤患者为癌症组,选取同期30例卵巢上皮性良性肿瘤患者为囊肿组及30例健康体检者为对照组,分别收集癌症组与囊肿组术前术后和对照组人员空腹静脉血3ml,采用酶联免疫吸附实验(ELISA)测定血清γ-干扰素(IFN-γ)和白细胞介素-4(IL-4)水平。结果术前癌症组患者IFN-γ水平低于囊肿组患者和对照组,IL-4水平高于囊肿组和对照组,Th1/Th2比值低于囊肿组和对照组,差异均有统计学意义(均P<0.05)。术后癌症组患者IFN-γ水平比术前上升,IL-4水平下降,Th1/Th2比值增加,差异均有统计学意义(均P<0.05);术前囊肿组患者与术后IFN-γ、IL-4水平及Th1/Th2比值相比,差异均无统计学意义(均P>0.05)。术后癌症组患者IFN-γ、IL-4水平及Th1/Th2比值与囊肿组患者相比,差异均无统计学意义(均P>0.05)。结论卵巢上皮性恶性肿瘤患者Th1细胞因子水平降低,Th2细胞因子水平升高,手术后得到纠正。检测恶性肿瘤患者的IFN-γ及IL-4,有助于判断恶性肿瘤预后,为免疫治疗提供参考。
Objective To investigate the expression of Th1 / Th2 cytokines in peripheral blood of patients with epithelial ovarian cancer before and after operation. Methods From January 2015 to March 2016, 30 cases of epithelial ovarian cancer patients admitted to Zhongshan Hospital Affiliated to Fudan University were selected as cancer group. Thirty patients with epithelial ovarian benign tumor were enrolled as cyst group and 30 healthy persons In the control group, 3 ml fasting venous blood was collected from the cancer group and cyst group before and after operation, and the levels of IFN-γ and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA) -4 (IL-4) levels. Results The level of IFN-γ in preoperative cancer group was lower than that in cyst group and control group, the level of IL-4 was higher in cyst group and control group, and the Th1 / Th2 ratio was lower in cyst group and control group All P <0.05). The level of IFN-γ in patients with postoperative cancer was significantly higher than that before operation, the level of IL-4 was decreased and the ratio of Th1 / Th2 was increased (all P <0.05). The preoperative cyst group and postoperative IFN-γ , IL-4 level and Th1 / Th2 ratio, there was no significant difference (all P> 0.05). There was no significant difference in the levels of IFN-γ, IL-4 and Th1 / Th2 between postoperative cancer group and cyst group (all P> 0.05). Conclusions The levels of Th1 cytokines and Th2 cytokines in patients with epithelial ovarian cancer are elevated and corrected after surgery. Detection of IFN-γ and IL-4 in malignant tumor patients is helpful to judge the prognosis of malignant tumor and provide a reference for immunotherapy.