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应用聚丙烯酰胺凝胶垂直板电泳（ＰＡＧＥ）分离和研究成体泥鳅心肌、骨骼肌、肝脏、卵巢、脑、眼、血清七种组织器官中的ＬＤＨ同工酶。结果表明：在电泳图谱负极端的相同部位，骨骼肌出现５条，肝脏有４条，脑组织１条ＬＤＨ同工酶区带；其余多数组织中均为３条酶区带；此外，近负极端，肝脏组织还有１条其他组织所没有的ＬＤＨ同工酶区带。这提示了泥鳅的ＬＤＨ同工酶可能是由ＬｄｈＡ、ＬｄｈＢ和ＬｄｈＣ３个基因座位编码的Ａ、Ｂ、Ｃ亚基组合成的四聚体。定量分析表明ＬＤＨ同工酶活力具有明显的组织特异性：血清、卵巢、肝脏等组织中ＬＤＨ５活性最强，而心肌和骨骼肌中则分别是ＬＤＨ２和ＬＤＨ３活性最强
Polyacrylamide gel electrophoresis (PAGE) was used to isolate and study LDH isozymes in cardiac, skeletal muscle, liver, ovary, brain, eye and serum of adult loach. The results showed that there were 5 skeletal muscle, 4 liver and 1 LDH isozymes in the same part of the negative end of the electropherogram. There were 3 bands in most of the tissues. In addition, nearly negative Extreme, liver tissue and LDH isoenzyme zone that one other organization does not have. This suggests that the LDH isozyme of loach may be a tetramer composed of A, B and C subunits encoded by LdhA, LdhB and LdhC3 loci. Quantitative analysis showed that LDH isozymes had obvious tissue specificity: LDH5 was the most active in serum, ovary and liver tissues, while LDH2 and LDH3 were the most active in myocardium and skeletal muscle