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目的:观察迷走神经兴奋对内毒素血症大鼠肝、肺组织中促炎因子(TNF-α)及抗炎因子皮质醇的影响,探讨胆碱能抗炎通路在炎症反应中的初步机制。方法:雄性 Wistar 大鼠,随机分为迷走神经刺激组、迷走神经切断后静注 LPS 组、假手术组和 LPS 组等4组。测定肝、肺 TNF-α、血浆皮质醇和丙氨酸氨基转移酶(ALT)含量。结果:静注 LPS 后,组织中 TNF 的含量显著升高(最高可达15倍),其中肝组织中 TNF 含量在1.5h 达到高峰,2h 回落,而肺组织中 TNF 含量在1h 达峰值;假手术组由于未注射 LPS,TNF 含量变化不明显,但肝、肺组织中 TNF 含量差异较大,肺组织中 TNF 含量明显低于肝组织,可能与 TNF 较多在肝脏合成有关。迷走神经切断
OBJECTIVE: To observe the effect of vagus nerve excitability on proinflammatory cytokine (TNF-α) and anti-inflammatory factor cortisol in liver and lung tissues of rats with endotoxemia and to explore the primary mechanism of cholinergic anti-inflammatory pathway in inflammation. Methods: Male Wistar rats were randomly divided into 4 groups: vagus nerve stimulation group, LPS group, sham operation group and LPS group after vagotomy. The liver and lung TNF-α, plasma cortisol and alanine aminotransferase (ALT) levels were determined. Results: After LPS injection, the content of TNF in the tissue increased significantly (up to 15 times). The content of TNF in liver tissue peaked at 1.5h and decreased at 2h, while the content of TNF in lung tissue peaked at 1h. In the operation group, the content of TNF did not change obviously due to the non-injection of LPS. However, the content of TNF in the liver and lung tissues was quite different. The content of TNF in the lung tissue was obviously lower than that in the liver tissue. Vagus nerve cut off