论文部分内容阅读
目的 了解梗阻性黄疸时小肠粘膜的病理改变。方法 无菌条件下结扎大鼠总胆管 ,取末端回肠观察其组织学改变。测量小肠粘膜的绒毛高度 ,绒毛面积和肠粘膜厚度 ;计算内脏细菌移位率。结果 在光镜下观察到总胆管结扎组肠粘膜萎缩 ,绒毛水肿 ,部分上皮细胞脱落 ;绒毛平均高度、绒毛平均面积和粘膜平均厚度减少。在电镜下观察到微绒毛稀疏 ,排列紊乱 ,内质网数目少且结构不完整 ;线粒体数目少 ,轻度肿胀 ,膜不清楚 ,嵴少而乱 ,内有空泡。肠系膜淋巴结、肝脏和脾脏的细菌移位率明显增加 ,移位的细菌主要是大肠杆菌和变形杆菌。结论 梗阻性黄疸时肠粘膜屏障功能严重受损 ,导致肠源性感染的发生。
Objective To understand the pathological changes of intestinal mucosa in obstructive jaundice. Methods Aseptic conditions, the total bile duct ligation of rats, take the terminal ileum to observe the histological changes. Measurement of villus height, villus area and intestinal mucosa thickness of intestinal mucosa; calculation of visceral bacterial translocation rate. Results Under the light microscope, intestinal mucosal atrophy, villus edema and some epithelial cells shedding were observed in the common bile duct ligation group. The average height of villus, the average area of villus and the average thickness of mucosa were decreased. Under the electron microscope, the microvilli were sparse and disordered, the number of endoplasmic reticulum was few and the structure was incomplete. The number of mitochondria was small, mild swollen, the membrane was not clear, the crest was less and disorderly, and there was vacuolization. Mesenteric lymph nodes, liver and spleen bacterial translocation rate was significantly increased, the shift of bacteria are mainly E. coli and Proteus. Conclusions Obstructive jaundice severely impaired intestinal barrier function, resulting in the occurrence of intestinal infection.