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目的探讨液相色谱-质谱法测定啤酒中3种伏马毒素的可行性。方法样品经乙腈提取,过Multi Sep~?211 Fum净化柱净化后,采用UPLC-MS/MS电喷雾正离子ESI+,以多离子反应监测(MRM)模式检测,外标法定量。结果 3种伏马毒素线性范围为2.001μg/L~100.12μg/L,线性相关系数在0.9990~0.9996之间,检出限在0.1~0.3μg/kg,3个不同水平的平均加标回收率在82.6%~91.5%间,相对标准偏差(RSD)均<6.08%。结论该方法有干扰少,操作简便,快速准确等优点,为检测啤酒中此类毒素提供了一种新的方法。
Objective To investigate the feasibility of three kinds of fumonisins in beer by liquid chromatography-mass spectrometry. Methods The samples were extracted with acetonitrile and cleaned up with Multi Sep ~? 211 Fum purification column. The UPLC-MS / MS electrospray positive ion ESI + was used for detection by multi-ion reaction monitoring (MRM) mode. Results The linear range of the three kinds of fumonisins was 2.001μg / L ~ 100.12μg / L, the linear correlation coefficient was between 0.9990 ~ 0.9996, the detection limit was between 0.1 ~ 0.3μg / kg, The relative standard deviations (RSDs) were both <6.08% between 82.6% and 91.5%. Conclusion The method has the advantages of less interference, simple operation, fast and accurate, which provides a new method for the detection of such toxins in beer.