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目的:探讨大蒜素对急性肺损伤大鼠的治疗作用及机制。方法:以雄性Wistar大鼠为对象通过颈动脉放血+气管LSP注射建立大鼠急性肺损伤模型,并通过静脉注射大蒜素予以治疗,以正常Wistar大鼠作为对照。术后通过病理检测、ELISA法等分析大蒜素疗效,并通过ELISA法、western blotting、PCR方法检测肺脏中Claudin-4表达变化。结果:经病理和ELISA检测显示大蒜素治疗组肺脏损伤情况明显好于急性肺损伤组,ELISA法、western blotting、PCR检测发现休克组大鼠肺脏Claudin-4表达较对照组明显降低,而以大蒜素治疗后Claudin-4表达可显著升高。结论:大蒜素可以减轻大鼠急性肺损伤,其机制可能是通过升高肺脏中Claudin-4的表达。
Objective: To investigate the therapeutic effect and mechanism of allicin on acute lung injury in rats. METHODS: The acute lung injury model was established in male Wistar rats through carotid artery blood transfusion and tracheal LSP injection. The rats were treated with intravenous allicin. Normal Wistar rats were used as controls. The effect of allicin was analyzed by pathology, ELISA, etc., and the expression of Claudin-4 in the lung was detected by ELISA, western blotting, and PCR. RESULTS: Pathological and ELISA showed that the lung injury in the allicin treatment group was significantly better than that in the acute lung injury group. The expression of Claudin-4 in the lung of the shock group was significantly lower than that in the control group by ELISA, western blotting, and PCR. Claudin-4 expression can be significantly increased after prime treatment. Conclusion: Allicin can reduce acute lung injury in rats. The mechanism may be through increasing the expression of Claudin-4 in the lung.