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目的检测深圳市福田区2009-2010年流感监测样本,分析甲型H1N1流感病毒的流行病学特点。方法采用实时荧光定量(Real Time RT-PCR)进行核酸检测和型别鉴定,同时进行病毒分离和血凝实验。结果 2009年8月-2010年12月共检测467份咽拭子样品,核酸检测阳性129份,阳性率为27.62%;病毒分离毒株101株,阳性率为21.63%。结论在2009年8月份甲型H1N1流感开始流行并成为流行的优势毒株,并持续到2010年3月份;5～59年龄人群甲型H1N1流感病毒分离率较高,而5岁以下及≥60岁年龄组分离率反而较低。
Objective To test the influenza surveillance samples from 2009 to 2010 in Futian District, Shenzhen and analyze the epidemiological characteristics of the influenza A (H1N1) virus. Methods Real-time RT-PCR was used for nucleic acid detection and type identification. Virus isolation and hemagglutination tests were performed simultaneously. Results A total of 467 throat swab samples were detected from August 2009 to December 2010, 129 of which were positive for nucleic acid. The positive rate was 27.62%. 101 strains of viruses were isolated, the positive rate was 21.63%. Conclusion Influenza A (H1N1) influenza became prevalent in August 2009 and became the predominant strain of endemic strain, which lasted until March 2010. The isolation rate of Influenza A (H1N1) virus was relatively high among those aged 5 to 59 years, while those below 5 years old and ≥60 Older age group separation rate is lower.