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为探讨外周血单个核细胞(PBMC)Th_1、Th_2类细胞因子的表达与食管鳞状细胞癌病理因素之间的关系,采用双抗体酶联免疫吸附法(ELISA)检测39例食管鳞状细胞癌患者外周血Th_1、Th_2类细胞因子的含量水平,同时以20例正常人作为健康对照。结果显示,食管鳞状细胞癌患者PBMC中IL-2、INF-γ表达明显低于对照组(P<0.05),IL-6表达明显高于对照组(P<0.05),存在Th_1/Th_2漂移现象;且TNM分期越晚,Th_1类细胞因子IL-2表达越弱,IL-6表达越强,存在显著差异性(P<0.05)。Th_1/Th_2漂移现象与肿瘤分化程度无明显相关性。提示食管鳞状细胞癌患者PBMC存在Th_2细胞优势,而Th_1类细胞因子表达减弱;Th_1/Th_2漂移现象与肿瘤的TNM分期有关。
To investigate the relationship between the expression of Th_1 and Th_2 cytokines in peripheral blood mononuclear cells (PBMCs) and the pathological factors of esophageal squamous cell carcinoma, 39 cases of esophageal squamous cell carcinoma were detected by enzyme-linked immunosorbent assay (ELISA) Th1, Th2 cytokines levels in peripheral blood of patients, and 20 normal subjects as healthy control. The results showed that the expression of IL-2 and INF-γ in PBMC of patients with esophageal squamous cell carcinoma was significantly lower than that of the control group (P <0.05), and the expression of IL-6 was significantly higher than that of the control group (P <0.05) (P <0.05). The later the TNM staging, the weaker the Th1 cytokine IL-2 expression and the stronger the IL-6 expression were (P <0.05). There was no significant correlation between Th_1 / Th_2 shift and tumor differentiation. The results suggest that PBMC of Thoracic esophageal squamous cell carcinoma has Th2 cell predominance and Th1 type cytokine expression weakened. Th1 / Th2 shift is related to TNM staging.