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1992年,Ｓａｎｏ等人将免疫测定技术与ＰＣＲ技术结合,创造了一种极其敏感的抗原分子检测技术,即免疫-ＰＣＲ。利用ＰＣＲ的敏感性来放大抗原抗体反应的特异性,使实验中只需要百个抗原分子的微量成份即可得到检测。这种灵敏度使免疫检测技术达到一个新高度。非常适合微量抗原的
In 1992, Sano et al. Combined immunoassay techniques with PCR techniques to create an extremely sensitive antigen molecule detection technique, immuno-PCR. The sensitivity of PCR is used to amplify the specificity of the antigen-antibody reaction so that only a trace amount of one antigen molecule can be detected in the experiment. This sensitivity brings immunoassay technology to new heights. Very suitable for trace antigens