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目的：研究热处理肿瘤细胞和小鼠脾淋巴细胞共同孵育后，淋巴细胞的杀伤毒性和增殖。方法：采用一般水浴加热处理小鼠Ｈ２２肝癌细胞后和脾淋巴细胞共培养，再将培养后的淋巴细胞攻击Ｈ２２肝癌细胞。结果：热处理后的肿瘤细胞能引起淋巴细胞增殖，并且能显著增强细胞毒性。结论：６５℃３０分钟热处理肿瘤细胞，能直接激活淋巴细胞并增强其对该肿瘤细胞的杀伤毒性长，毒性增强。提示热处理后的瘤细胞能增强和激活脾淋巴细胞的细胞毒性。表１热处理瘤细胞诱导的淋巴细胞的杀伤活性组别共同孵育时间（小时）和淋巴细胞杀伤率（％）１２２４４８不加瘤组６．３±２．０１０．４±１．０９．８±２．１未处理瘤组１０．１±３．６２７．０±１．５２２．１±１．７热处理瘤组２４．７±３．９＊４１．０±１．４＊５７．６±３．１＊Ｐ＜０．０１三、淋巴细胞的增殖：淋巴细胞与热处理Ｈ２２肝癌细胞共同孵育的时间段里，淋巴细胞表现出不同的增殖数（见表２）。表２热处理瘤细胞对淋巴细胞的扩增数的影响组别共同孵育时间（小时）和淋巴细胞数（×１０３）１２２４４８不加瘤组２．２±０．８２．７±０．７２．９±０．５未处理瘤组２．１±０．３２．３±０．２２．５±０．４热处理瘤组２．５±０．８?
Objective: To study the killing toxicity and proliferation of lymphocytes after co-incubation of heat-treated tumor cells and mouse spleen lymphocytes. METHODS: H22 hepatoma cells were treated with common water bath and co-cultured with spleen lymphocytes, and then the cultured lymphocytes were challenged with H22 hepatoma cells. Results: Heat treated tumor cells can cause lymphocyte proliferation and can significantly enhance cytotoxicity. Conclusion: Heat treatment of tumor cells at 65°C for 30 minutes can directly activate lymphocytes and enhance its long-term cytotoxicity and toxicity. It is suggested that the heat-treated tumor cells can enhance and activate the cytotoxicity of splenic lymphocytes. Table 1 Killing activity of lymphocytes induced by heat-treated tumor cells Group co-incubation time (hours) and lymphocyte killing rate (%) 122448 tumor-free group 6.3±2.010.4±1.09.8±2 .1 untreated tumor group 10.1±3.627.0±1.522.1±1.7 heat-treated tumor group 24.7±3.9*41.0±1.4*57.6±3. 1*P<0.01 3. Proliferation of Lymphocytes: In the period of incubation of lymphocytes with heat-treated H22 hepatoma cells, lymphocytes showed different proliferation numbers (see Table 2). Table 2 Effect of heat-treated tumor cells on the number of lymphocyte expansions Co-incubation time (hours) and number of lymphocytes (x 103) 122,448 tumor-free group 2.2±0.82.7±0.72.9 In the ±0.5 untreated tumor group, 2.1±0.32.3±0.22.5±0.4 heat-treated tumor group 2.5±0.8?