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采用固相萃取-电导抑制离子色谱法测定了萝芙木中常见有机酸和无机阴离子。以Ionpac AS11为分离柱,Ionpac AG11为保护柱,萝芙木样品过IC SPE-C18前处理柱去除杂质和有机物,在确定的色谱条件下,柱温30℃,淋洗液为7.5 mmol/L Na OH溶液,流速为0.7 m L/min,电导检测器-离子色谱法直接测定样品,方法的相关系数在0.9987~0.9999之间,样品回收率在83.0%~100.8%之间,RSD<5%。方法适用于中草药中常见有机酸和无机阴离子含量的测定。
The common organic acids and inorganic anions in Rauvolfia were determined by solid-phase extraction and conductivity-suppressed ion chromatography. Ionpac AS11 was used as the column and Ionpac AG11 as the guard column. The samples of Rauwolfi were pre-treated with IC SPE-C18 to remove impurities and organic compounds. The column temperature was 30 ℃ and the elution volume was 7.5 mmol / L Na OH solution with a flow rate of 0.7 m L / min. The correlation coefficient of the method was between 0.9987 and 0.9999. The recoveries of the samples ranged from 83.0% to 100.8% with RSD <5% . The method is suitable for the determination of common organic acids and inorganic anions in Chinese herbal medicine.