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研究茶多酚对慢性不可预知应激抑郁模型中小鼠的抗抑郁作用及机制。建立慢性不可预知性刺激小鼠抑郁模型,通过小鼠旷场实验、强迫游泳实验和悬尾实验中对茶多酚的抗抑郁作用进行研究;并采用分光光度法检测小鼠脑组织中丙二醛(MDA)的水平及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活性,同时测定小鼠脑组织中5-羟色胺(5-hydroxytryptamine,5-HT)以及去甲肾上腺素(Norepinephrine,NE)的含量。给予茶多酚低高2个剂量组小鼠在旷场实验中的爬行格数与直立次数较模型组显著增加(#P<0.05,△P<0.001);小鼠在强迫游泳实验和悬尾实验中的不动时间较模型组明显减少(#P<0.05,△P<0.001;#P<0.05,△P<0.05),显示造模成功;此外,茶多酚可显著抑制脑组织中MDA的含量增加,并上调SOD和GSH-Px的活性,明显提升小鼠脑组织中5-HT和NE的含量。茶多酚对慢性不可预知应激引起的小鼠抑郁具有改善作用,其机制可能与上调脑部5-HT和NE的含量以及抑制脑组织氧化应激有关。
To study the antidepressant effect and mechanism of tea polyphenols on chronic unpredictable stress depression model mice. To establish a chronic unpredictable model of depression in mice and to study the antidepressant effect of tea polyphenols in open-field test, forced swimming test and tail-suspending test in mice. The level of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the brain tissue of mice were measured. The levels of 5-hydroxytryptamine (5-HT ) And norepinephrine (NE) content. Compared with the model group, the number of creeping cells and the number of erections in two low-dose groups of tea polyphenols (P <0.05, P <0.001) were significantly higher than those in the untreated group The immobility time in the experiment was significantly lower than that in the model group (#P <0.05, P <0.001; #P <0.05, P <0.05) Increased the activity of SOD and GSH-Px, and significantly increased the content of 5-HT and NE in mouse brain tissue. Tea polyphenols can ameliorate depression in mice induced by chronic unpredictable stress, and its mechanism may be related to the up-regulation of brain 5-HT and NE content and the inhibition of oxidative stress in brain tissue.