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目的观察糖基化终产物(AGEs)对胎鼠海马神经前体细胞(NPCs)增殖和分化的影响。方法分离孕14.5d胎鼠海马,有限稀释法进行单克隆体外培养,分为不同浓度AGEs-牛血清白蛋白(BSA)组和对照组。分别用无血清NPCs培养基和胎牛血清促其增殖和诱导分化。MTT法和免疫荧光双标法结合激光共聚焦成像分别观察不同浓度AGEs对NPCs增殖和分化的影响。结果AGEs-BSA(50～400)mg/L干预后第3天和第7天,吸光度(A570nm)显著降低(P<0.01);AGEs-BSA(50～400)mg/L干预7d后,NeuN阳性细胞率显著低于对照组(P<0.05)。结论糖基化终产物抑制胎鼠NPCs的增殖和向神经元的分化。
Objective To investigate the effect of advanced glycation end products (AGEs) on the proliferation and differentiation of fetal rat hippocampal neural precursor cells (NPCs). Methods Fetal rat hippocampus was isolated from pregnant 14.5 day embryos and cultured in vitro by limiting dilution method. The cells were divided into AGEs-BSA group and control group. Respectively with serum-free NPCs and fetal bovine serum to promote proliferation and differentiation. MTT assay and immunofluorescence double labeling method combined with confocal laser scanning microscope were used to observe the effects of different concentrations of AGEs on the proliferation and differentiation of NPCs. Results The absorbance (A570nm) of AGEs-BSA (50-400mg / L) was significantly decreased on the 3rd and 7th day (P <0.01) The positive cell rate was significantly lower than that of the control group (P <0.05). Conclusion The advanced glycation end products can inhibit the proliferation and differentiation of NPCs in fetal rat NPCs.