AIM:To study the antitumor and immunomodulatory activityof resveratrol on experimentally implanted tumor of H22 inBalb/c miceMETHODS:The cytotoxicity of peritoneal macrophages (Mφ) against H22 cells was measured by the radioactivity of[~3H]TdR assay,mice with H22 tumor were injected withdifferent concentrations of resveratrol,and the inhibitoryrates were calculated and IgG contents were determined bysingle immunodiffusion method,the plaque forming cell(PFC) was measured by improved Cunningham method,thelevels of serum tumor necrosis factor-α (TNF-α) weremeasured by cytotoxic assay against L929 cells.RESULTS:Resveratrol 2.5 mg·L~(-1),5.0 mg·L~(-1),10.0 mg·L~(-1),20.0 mg·L~(-1) (E:T=10:1,20:1) promoted the cytotoxicity ofM φ against H22 cells.Resveratrol ip 500 mg·kg~(-1),1 000mg·kg~(-1) and 1 500 mg·kg~(-1) could curb the growth of theimplanted tumor of H22 in mice.The inhibitory rates were31.5%,45.6% and 48.7%,respectively (P<0.05),whichcould raise the level of serum IgG and PFC response tosheep red blood cell.Resveratrol 1 000 mg·kg~(-1) and 1 500mg·kg~(-1) and BCG 200 mg·kg~(-1) ip could increase theproduction of serum TNF-α in mice H22 tumor.However,the effect of resveratrol was insignificant (P>0.05).CONCLUSION:Resveratrol could inhibit the growth of H22tumor in Balb/c mice.The antitumor effect of resveratrolmight be related to directly inhibiting the growth of H22cells and indirectly inhibiting its potential effect on nonspecifichost immunomodulatory activity. AIM: To study the antitumor and immunomodulatory activity of resveratrol on experimentally implanted tumor of H22 inBalb/c miceMETHODS: The cytotoxicity of peritoneal macrophages (Mφ) against H22 cells was measured by the radioactivity of [~3H]TdR assay,mice with H22 tumor were ELISA withdifferent concentrations of resveratrol,and the inhibitoryrates were calculated and IgG contents were determined bysingle immunodiffusion method,the plaque forming cell(PFC) was measured by improved Cunningham method,thelevels of serum tumor necrosis factor-α (TNF-α) weremeasured by cytotoxic Assay against L929 cells.RESULTS: Resveratrol 2.5 mg·L -1, 5.0 mg·L -1, 10.0 mg·L -1, 20.0 mg·L -1 (E: T=10:1,20:1) promoted the cytotoxicity of M φ against H22 cells. Resveratrol ip 500 mg·kg -1 , 1 000 mg·kg -1 and 1 500 mg·kg -1 ) could curb the growth of the implanted tumor of H22 in mice. The inhibitory rates were 31.5%, 45.6% and 48.7%, respectively, (P<0.05), which could raise the level of serum IgG and PFC response tosheep red blood cell.Resveratrol 1 000 mg·kg -1 and 1 500 mg·kg -1 and BCG 200 mg·kg -1 ip raised the production of serum TNF-α in mice H22 tumor.However, the effect of resveratrol was insignificant (P>0.05).CONCLUSION:Resveratrol could inhibit the growth of H22 tumor in Balb/c mice.The antitumor effect of resveratrolmight be related to directly inhibiting the growth of H22 cells and indirectly inhibiting its Potential effect on nonspecifichost immunomodulatory activity.