本文试图用整体吸入染毒法分析肺灌洗液中肺巨噬细胞的数量、死亡率和非细胞成分的变化,以评价低浓度三氯化磷(PCl_3)对肺细胞毒作用。实验证明PCl_3一次急性吸入890.59mg/m~3(高毒组),以上指标均有明显改变,井出现在病理改变之前,反映低浓度PCl_3对肺细胞有轻度毒作用,认为此方法比短期体外试验法更能反映毒物对机体的作用。 In this paper, we attempted to analyze the changes of lung macrophages in lung lavage fluid and the changes of non-cellular components with the method of inhalation and inhalation to evaluate the cytotoxicity of PCl_3 to lung cells. Experiments show that acute inhalation of PCl_3 890.59mg / m ~ 3 (high toxicity group), the above indicators were significantly changed, well appeared before the pathological changes, reflecting the low concentration of PCl_3 on lung cells with mild toxic effects, that this method than short-term in vitro Test method can better reflect the role of poison on the body.