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目的观察缺氧缺血性脑损伤(HIBD)新生大鼠脑室管膜下区Foxg1基因与p21基因表达的动态变化,探讨二者的相互关系。方法采用R ice法制作新生大鼠HIBD动物模型,假手术组作为对照,分别在术后第3、7、14、21、28天处死,取其左侧大脑半球,应用原位杂交法测定HIBD模型组与对照组新生大鼠Foxg1基因与p21基因在脑室管膜下区(SVZ)不同时间点表达水平。结果 1.HIBD后SVZ区Foxg1基因3 d时与假手术组比较已明显升高(P<0.05),7 d时表达达高峰,此后逐渐下降,各时间点表达水平均较假手术组高(Pa<0.05),假手术组各时间点Foxg1表达水平变化不大。2.HIBD后p21基因3 d时表达较假手术组明显升高(P<0.05),7 d时表达水平最低,此后又逐渐升高,各时间点表达水平均较假手术组高(Pa<0.05),假手术组也有升高趋势。3.二组p21基因与Foxg1基因均呈负相关。结论 HIBD可以促进新生大鼠脑组织SVZ区Foxg1基因表达,HIBD后SVZ区细胞凋亡增加,p21基因表达上调。Foxg1基因可能是p21基因的上游调节基因。
Objective To observe the dynamic changes of Foxg1 gene and p21 gene expression in the subventricular zone of neonatal hypoxic-ischemic brain damage (HIBD) -related neonatal rats and to explore their relationship. Methods The HIBD model of neonatal rats was made by R ice method. The sham operation group was taken as the control. The rabbits were sacrificed on the 3rd, 7th, 14th, 21st and 28th day respectively. The hemisphere of the left hemisphere was taken and the in situ hybridization The expression levels of Foxg1 and p21 in the subventricular zone (SVZ) at different time points in neonatal rats in model group and control group. After HIBD, Foxg1 gene in SVZ was significantly increased (P <0.05) at 3 d and peaked at 7 d, then decreased gradually, and the expression level of Foxg1 at each time point was higher than that of sham operation group Pa <0.05). There was no significant change in Foxg1 expression in sham operation group at each time point. The expression of p21 gene at 3 d in HIBD group was significantly higher than that in sham-operation group (P <0.05), and the expression level of p21 gene was the lowest at 7 d and then gradually increased at each time point (P < 0.05), sham operation group also increased. The two groups of p21 gene and Foxg1 gene were negatively correlated. Conclusion HIBD can promote Foxg1 gene expression in SVZ of neonatal rat brain, increase apoptosis of SVZ after HIBD, and up-regulate p21 gene expression. Foxg1 gene may be upstream regulator of p21 gene.