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目的 研究纯化培养的大鼠星型胶质细胞的IP3 和非IP3 敏感钙库的特征和相关性。方法 用显微荧光测量技术监测单个星型胶质细胞内钙信号的动态变化 ,用IP3 敏感钙库和非IP3 敏感钙库的激活剂MCh和CAF及线粒体解偶剂FCCP刺激胶质细胞 ,观察它们胞内钙信号的影响。结果 在大部分细胞上 ,两种钙库中的任何一种被削弱或完全排空后 ,另一种钙库被激活所诱发的 [Ca2 + ] I 的升高幅度不受影响或仅被轻度抑制 ;而在少数细胞上 ,一种钙库被削弱或完全排空后 ,另一种钙库被激活所诱发的 [Ca2 + ] I 的升高幅度明显降低。结论 研究显示星型胶质细胞内的钙库包括 :IP3敏感和非IP3敏感的两种内质网钙库 ,它们相对独立或密切相关
Objective To study the characteristics and correlation of IP3 and non-IP3-sensitive calcium pools in purified cultured rat astrocytes. Methods The dynamic changes of intracellular calcium signaling in single astrocytes were monitored by fluorescence microscopy. The activation of glial cells was stimulated by MCh and CAF in IP3-sensitive and non-IP3-sensitive calcium stores and by FCCP. Their effect on intracellular calcium signaling. Results On most cells, the increase in [Ca2 +] I induced by activation of another calcium pool was not affected or was diminished only after either of the two calcium pools were depleted or completely emptied Degree inhibition; in a few cells, a calcium library is weakened or completely emptying, the activation of another calcium library induced [Ca2] I increased significantly reduced. Conclusions Studies have shown that astrocytes within the calcium library include: IP3-sensitive and non-IP3-sensitive two endoplasmic reticulum calcium stores, which are relatively independent or closely related