目的研究消化系恶性肿瘤（ＤＭＴ）患者血清与腹水中内源性ＩＬ２，ＩＬ６，ＩＬ８，ＴＮＦα和ＩＦＮγ的生物学活性．方法应用ＥＬＩＳＡ法检测了１５例ＤＭＴ患者（肝癌１１例，胆总管癌１例，胰腺癌１例，胃癌１例，直肠癌１例）血清与腹水中５种细胞因子活性，并与６例肝硬变（ＬＣ）患者和８例正常成人进行了比较分析．结果ＤＭＴ患者血清ＩＬ２，ＩＬ６的生物学活性显著低于ＬＣ（Ｐ＜００５）；腹水中ＩＬ２，ＩＬ８活性显著低于ＬＣ组（Ｐ＜００１），而ＩＬ６和ＩＦＮγ活性则高于ＬＣ组（Ｐ＜００１，００５）．ＤＭＴ患者血清中ＩＬ６，ＩＬ８活性明显高于正常成人组（Ｐ＜００５）；ＩＬ２，ＩＦＮγ则低于正常成人组，但缺乏显著性．肝癌血清和腹水中ＩＬ２活性显著高于非肝癌组（Ｐ＜００５）；而ＩＬ６活性则相对降低（Ｐ＜００５）．结论恶性肿瘤患者血清中ＩＬ２和ＩＦＮγ活性低于正常人，是ＤＭＴ患者抗肿瘤免疫功能缺陷的标志．ＩＬ６对于预测ＤＭＴ患者的预后具有重要的意义 Objective To study the biological activities of endogenous IL-2, IL-6, IL-8, TNFα and IFNγ in serum and ascites of patients with digestive malignancy (DMT). Methods The activity of 5 cytokines in serum and ascites in 15 patients with DMT (11 cases of hepatocellular carcinoma, 1 case of common bile duct cancer, 1 case of pancreatic cancer, 1 case of gastric cancer, and 1 case of rectal cancer) was detected by ELISA and compared with 6 cases of liver disease. A comparative analysis was made between patients with hardened (LC) and 8 normal adults. Results The biological activity of IL2 and IL6 in serum of DMT patients was significantly lower than that of LC (P<005). The activity of IL2 and IL8 in ascites was significantly lower than that in LC group (P<001). The activity of IL-6 and IFNγ was higher than that of LC group (P<001,005). The serum levels of IL-6 and IL-8 in DMT patients were significantly higher than those in the normal adult group (P<0.05). IL-2 and IFNγ were lower than those in the normal adult group, but they lacked significance. IL  2 activity in serum and ascites of hepatocellular carcinoma was significantly higher than that in non-hepatocellular carcinoma group (P<005), while IL6 activity was relatively decreased (P<005). Conclusion The serum levels of IL-2 and IFN-γ in patients with malignant tumors are lower than those in normal subjects, which is a sign of anti-tumor immune function defects in DMT patients. IL-6 plays an important role in predicting the prognosis of patients with DMT