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目的研究海水淹溺性肺水肿对大鼠颅脑创伤后血脑屏障通透性的影响。方法 32只雄性SD大鼠随机均分为海水淹溺(A)组、颅脑创伤合并淡水淹溺(B)组、单纯创伤(C)组和正常对照(D)组。采用侧方液压打击颅脑伤合并海水淹溺大鼠模型,用胶体金示踪血脑屏障通透性的变化。伤后6 h取大鼠伤区脑组织做电镜检查,了解胶体金颗粒透过血脑屏障入脑情况。结果 A组可见神经元细胞线粒体肿胀,髓鞘崩解,胶质细胞肿胀。神经元细胞染色体边集,甚至出现核固缩。脑微血管周围间隙扩大,胞饮活动增强。与B、C组相比,A组电镜可见有更多胶体金颗粒透过血脑屏障入脑。结论海水淹溺性肺水肿可增加血脑屏障的通透性,加重创伤性脑水肿。
Objective To study the influence of seawater drowning pulmonary edema on permeability of blood-brain barrier after traumatic brain injury in rats. Methods Thirty - two male Sprague - Dawley rats were randomly divided into three groups: seawater drowning (A) group, craniocerebral trauma combined with freshwater drowning (B) group, simple trauma group (C) and normal control (D) group. Lateral hydraulic pressure was used to combat the rat model of craniocerebral injury combined with seawater drowning, and the permeability of the blood-brain barrier was traced with colloidal gold. 6 h after injury to take rat brain tissue injury area electron microscopy to understand the colloidal gold particles into the brain through the blood-brain barrier situation. Results Group A showed mitochondria swelling, myelin disintegration and glial swelling in neurons. Neuronal cell chromosome edge sets, and even nuclear pyknosis. Brain microvascular enlargement around the gap, increased pinch activity. Compared with group B and group C, there were more colloidal gold particles penetrating the blood-brain barrier into brain in group A. Conclusion Drowning seawater edema can increase the permeability of blood-brain barrier and aggravate traumatic brain edema.