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目的了解湖南地区新生儿感染肺炎克雷伯菌染色体介导喹诺酮类耐药基因gyrA基因的流行情况。方法采用PCR法对20株肺炎克雷伯菌进行gyrA基因检测,采用法国生物梅里埃公司生产的VITEK2-COMPACT全自动细菌鉴定检测16种抗菌药物的体外抗菌活性,PCR扩增产物经纯化后测序并进行序列分析。结果 gyrA基因耐药决定区的突变,其中17株都有第83位的改变,3株发现有第87位的改变。结论本省儿童感染肺炎克雷伯菌染色体介导喹诺酮类耐药基因gyrA基因突变情况十分普遍,监测其突变频率、位点对指导临床用药及耐药机制研究十分重要。
Objective To investigate the prevalence of quinolone-resistant gene gyrA gene in neonates infected with Klebsiella pneumonia in Hunan Province. Methods The gyrA gene of 20 strains of Klebsiella pneumoniae was detected by PCR method. The antibacterial activity of 16 antibacterial agents was detected by VITEK2-COMPACT automatic bacterial identification method. The PCR products were purified and sequenced And sequence analysis. Results The gyrA gene resistance was determined to be a mutation in the region, of which 17 had a 83rd change and 3 had a 87th change. Conclusion The gyrA gene mutation of quinolone-resistant gene is most common in children infected with Klebsiella pneumoniae in our province. It is very important to monitor the mutation frequency and site to guide clinical drug use and drug resistance mechanism.