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从正常和冷冻大鼠肝脏中分离出线粒体。用氧电极法测定鼠肝线粒体呼吸功能,包括ADP:O比值(P/O值),呼吸控制率(RCR),以[~3H]-Leu参入为指标,观察线粒体内蛋白质合成;以SDS-聚丙烯酰胺凝胶电泳方法观察线粒体内膜蛋白质的变化。结果表明:冷适应2、4周时P/O值和RCR降低,6周时两指标恢复至正常水平。冷适应1,2,4周时线粒体蛋白质合成明显降低。冷适应2,3周时线粒体内膜蛋白质电泳图谱呈现明显变化,冷适应组线粒体内膜有新的蛋白质条带出现。
Mitochondria were isolated from normal and frozen rat livers. Mitochondrial respiratory function, including ADP: O ratio (P / O) and respiratory rate (RCR), was measured by the oxygen electrode method. The mitochondrial protein synthesis was observed using [~ 3H] The changes of mitochondrial inner membrane protein were observed by polyacrylamide gel electrophoresis. The results showed that the P / O value and RCR decreased at 2 and 4 weeks after cold acclimation, and returned to normal after 6 weeks. Cold adaptation mitochondrial protein synthesis was significantly reduced at 1, 2 and 4 weeks. Cold adaptation 2, 3 weeks when the mitochondrial inner membrane protein electrophoresis showed significant changes in the cold adapted mitochondrial membrane of a new protein bands appear.