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目的研究低浓度气态苯吸入对大鼠学习记忆等神经行为的影响,并探讨低浓度苯吸入对大鼠血象的改变。方法SD雄性大鼠随机分4组,每组10只,低、中、高剂量组苯染毒浓度分别为4 mg/m3、6 mg/m3和10 mg/m3,对照组吸入空气。采用静式吸入染毒法,4 h/d,5 d/w,持续染毒4 w,染毒结束后进行学习记忆等神经行为学测试、血象、嗜多染红细胞微核实验。结果 Morris水迷宫定位航行实验,高剂量组大鼠d 3、d 4的逃避潜伏期明显高于对照组(P<0.05),空间探索实验的目标象限时间明显低于对照组(P<0.05)。高剂量组大鼠的后肢撑力指数明显高于对照组(P<0.05)。低、中、高剂量组白细胞计数明显低于对照组(P<0.05)。结论低剂量、短时间暴露苯可损害大鼠学习记忆及运动功能,引起外周血白细胞计数降低。
Objective To study the effect of inhaling low concentration gaseous benzene on learning and memory and other neurological behaviors in rats and to explore the changes of blood parameters in rats induced by low concentration benzene inhalation. Methods SD male rats were randomly divided into 4 groups with 10 rats in each group. The benzene concentrations in low, medium and high dose groups were 4 mg / m3, 6 mg / m3 and 10 mg / m3, respectively. The control group inhaled air. The rats were exposed to static inhalation for 4 h / d and 5 d / w for 4 w. The neurobehavioral tests, such as learning and memory, were performed after the exposure. Blood micronucleus test was performed on the blood and polychromatic erythrocytes. Results In the Morris water maze test, the escape latency of d 3 and d 4 in the high dose group was significantly higher than that in the control group (P <0.05). The target quadrant time of the space exploration experiment was significantly lower than that of the control group (P <0.05). The hindlimb support index of high dose group was significantly higher than that of control group (P <0.05). Low, medium and high doses of white blood cell count was significantly lower than the control group (P <0.05). Conclusion Low-dose and short-time exposure of benzene can impair the learning, memory and motor function of rats and lead to the decrease of peripheral white blood cell count.