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目的评价优化核酸疫苗(pVAX1-IL-4/SYN-B)预防接种对小鼠MPTP损害的免疫保护作用。方法将C57BL/6小鼠随机分为正常对照组、PBS模型组、空质粒模型组、优化核酸疫苗模型组4组,分别在小鼠双后肢胫前肌注射PBS、PBS、空质粒、pVAX1-IL-4/SYN-B各100μl,共3次。末次注射3周后,后3组注射MPTP构建慢性帕金森病小鼠模型,正常对照组注射同体积的PBS。末次给药后观察小鼠行为学变化;免疫组织化学、Western blot检测小鼠黑质部酪氨酸羟化酶(tyrosine hydroxylase,TH)表达情况;RT-PCR检测小鼠黑质TNF-α、IL-1β的mRNA水平。结果与PBS模型组、空质粒模型组相比,优化核酸疫苗模型组小鼠运动症状明显改善;TH丢失减少;TNF-α、IL-1β的mRNA水平表达降低;差异有统计学意义。结论优化核酸疫苗预防接种对小鼠的慢性MPTP损害有一定的神经保护及抗炎作用。
Objective To evaluate the immunoprotective effect of vaccination with optimized nucleic acid vaccine (pVAX1-IL-4 / SYN-B) on MPTP damage in mice. Methods C57BL / 6 mice were randomly divided into four groups: normal control group, PBS model group, empty plasmid model group and optimized nucleic acid vaccine model group. PBS, PBS, empty plasmid, pVAX1- IL-4 / SYN-B each 100μl, a total of 3 times. Three weeks after the last injection, mice in the latter three groups were injected with MPTP to establish a chronic Parkinson’s disease mouse model, and the normal control group was injected with the same volume of PBS. Immunohistochemistry and Western blot were used to detect the expression of tyrosine hydroxylase (TH) in the substantia nigra of mice. The levels of TNF-α, IL-1β mRNA levels. Results Compared with the PBS group and the empty plasmid group, the mice in the optimized vaccine group showed significant improvement in motor symptoms, reduced TH loss, and decreased the expression of TNF-α and IL-1β mRNA. The difference was statistically significant. Conclusion The optimized DNA vaccination has some neuroprotective and anti-inflammatory effects on chronic MPTP damage in mice.