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非放射性的生物素（Ｂｉｏｔｉｎ）和地高辛（Ｄｉｇｏｘｉｇｅｎｉｎ）标记的乙肝病原基因探针（Ｂｉｏ－ＨＢＶＤＮＡ和Ｄｉｇ－ＨＢＶＤＮＡ探针），特异性强，灵敏度高，最低检出值分别为１ｐｇ和０．５ｐｇ。３２０份血清检测了ＨＢＶＤＮＡ和ＨＢｅＡｇ，并与α－_（３２）Ｐ标记的探针检测结果相比较，它们的特异性分别为９６．５％、９８．１％和８８．９％；灵敏度分别为８１．７％、８６．７％和６１．７％；符合率则为９３．８％、９５．９％和８３．８％。应用Ｄｉｇ－ＨＢＶＤＮＡ探针，作斑点杂交试验，在乙肝患者血清、白细胞、尿、粪、唾液以及旅客列车的桌面、车箱门把手、厕所门把手和水笼头上，均可检得阳性结果。我所研制的非放射性乙型肝炎病原基因诊断试剂，为科学研究和临床检测提供了极为有用的工具。
Non-radioactive Biotin and Digoxigenin-labeled HBeAg probes (Bio-HBVDNA and Dig-HBVDNA probes) are highly specific and sensitive with minimum detectable values of 1 pg and 0 .5pg. 320 serum samples were tested for HBVDNA and HBeAg, and their specificity was 96.5%, 98.1% and 88.9%, respectively, compared with the results of α-32 probe. The sensitivity was 81.7%, 86.7% and 61.7% respectively. The coincidence rates were 93.8%, 95.9% and 83.8% respectively. The Dig-HBVDNA probe was used for spot blot test to detect positive results on the surface of hepatitis B patients’ serum, white blood cells, urine, feces and saliva, and on the passenger train’s tabletop, the trunk door handle, the toilet door handle and the faucet. I developed non-radioactive hepatitis B pathogen diagnostic reagents, for scientific research and clinical testing provides a very useful tool.