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目的探讨人精液一氧化氮(NO)与总蛋白(TP)、白蛋白(Alb)和转铁蛋白(Tf)含量的关系。方法参照WHO标准方法,进行精液常规分析。采用镀铜镉还原荧光法检测NO代谢产物硝酸盐(NO-3),用双缩脲法检测TP,溴甲酚绿法测定Alb,用免疫散射比浊法检测Tf含量。结果不育活率异常组、少精子和无精子症组的NO含量明显高于正常生育组,TP、Alb和Tf含量明显低于正常生育组,两组之间存在高度显著性差异(P<0.01),不育组NO含量与TP、Alb和Tf含量呈显著性负相关(r=-0.88、r=-0.98、r=-0.68,P均<0.05)。结论精液蛋白质含量测定有助于精子质量的评价,对男性不育症的诊治有一定的指导意义,NO对精子运动能力及蛋白质的分泌利用有抑制作用,这对不育症的机制研究有重要价值。
Objective To investigate the relationship between nitric oxide (NO) and total protein (TP), albumin (Alb) and transferrin (Tf) in human semen. Methods According to WHO standard methods, routine sperm analysis. The NO metabolite nitrate (NO-3) was detected by copper-cadmium reduction fluorescence method. TP was measured by the biuret method and Alb was determined by the bromocresol green method. The Tf content was detected by the immunostaining turbidimetry. Results The NO level in infertile infertility group, oligospermia and azoospermia group was significantly higher than that in normal fertility group, while the contents of TP, Alb and Tf were significantly lower than those in normal fertility group (P < 0.01). The content of NO in infertile group was negatively correlated with TP, Alb and Tf (r = -0.88, r = -0.98, r = -0.68, P <0.05). Conclusion The determination of sperm protein content contributes to the evaluation of sperm quality, which has some guiding significance for the diagnosis and treatment of male infertility. NO can inhibit sperm motility and protein secretion, which is important for the study of the mechanism of infertility value.