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体外淋巴细胞转化率的测定,是观察细胞免疫机能的重要方法之一。常用的Eagle培养基需要13种氨基酸、多种维生素及无机盐类,制作手续复杂,试剂昂贵,使此项检查在很多单位不易开展。采用Hank液加水解乳旦自作培养基虽较简易,仍需试剂较多,而且培养效果不够理想。半年多来,我们试用维生素B_(12)加小牛血清作培养基测定淋巴细胞转化率,制作手续简便,效果良好,现简介如下。
In vitro lymphocyte transformation rate determination is one of the important ways to observe the cellular immune function. Commonly used Eagle medium requires 13 kinds of amino acids, a variety of vitamins and inorganic salts, the production process is complex, expensive reagents, so that the inspection is not easy to carry out in many units. Using Hank solution plus hydrolyzed milk for self-made medium is relatively simple, still need more reagents, and the culture effect is not ideal. More than six months, we try vitamin B_ (12) plus calf serum for determination of lymphocyte transformation rate, the production process is simple, the effect is good, are as follows.