论文部分内容阅读
以３种方法（洗脱法、浇入法、贴膜法）对含抑菌成分的药品微生物限度检查中薄膜过滤后细菌、霉菌、酵母菌数检查方法进行了验证。现行的滤膜洗脱法不能正确反映被检品染菌量，平均回收率仅为１３％ 。而贴膜法能定量反映被检品的染菌量，平均回收率达９７％ ，且操作方便、步骤少；由于采用了陶瓦盖，所得菌落饱满、凸现，便于观察、计数和分类定性。
Three methods (elution method, pouring method and film method) were used to check the method of bacterial, mold and yeast count after the membrane filtration in the limit test of microorganisms containing antibacterial components. The current membrane elution method does not accurately reflect the amount of contaminated bacteria in the sample, with an average recovery of only 13%. The film method can quantitatively reflect the amount of bacteria in the seized product, the average recovery rate of 97%, and easy to operate, less steps; due to the use of Tao tile cover, the resulting colonies full, highlights, easy to observe, count and classification qualitative.