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为了探析辣椒(Capsicum annuum)下胚轴不定根发生的分子机制,采用转录组学方法研究了不定根发生诱导期和启动期的差异表达基因。结果表明:在AR24/AR0、AR120/AR0和AR120/AR24比较组中分别鉴定到4 991、6 658和4 128个差异表达基因,其中1 321个为3个比较组所共有。对差异表达基因进行GO和KEGG显著性富集分析,发现辣椒下胚轴不定根发生的诱导期主要涉及激素应答、激素信号转导、细胞壁降解与重塑,启动期则是DNA复制、转录与翻译、细胞分裂和细胞壁合成等;纵观两个时期,不定根的发生由生长素、细胞分裂素、乙烯和油菜素内酯相关基因调控,并且与GRAS、AP2、ERF、LBD、SRS、WRKY家族转录因子表达密切相关。由上可知,辣椒下胚轴不定根发生是由一系列信号分子参与且极其复杂的器官发生过程。
In order to investigate the molecular mechanism of adventitious root formation in hypocotyls of Capsicum annuum, we used transcriptomics to study differentially expressed genes at induction and initiation stages of adventitious roots. The results showed that 4 991,6 658 and 4 128 differentially expressed genes were identified in the AR24 / AR0, AR120 / AR0 and AR120 / AR24 comparison groups respectively, of which 1 321 were shared by 3 comparison groups. Significant enrichment analysis of the differentially expressed genes by GO and KEGG revealed that induction of adventitious root of pepper hypocotyl mainly involved in hormone response, hormone signal transduction, cell wall degradation and remodeling, and initiation of DNA replication, transcription and translation , Cell division and cell wall synthesis. During the two periods, adventitious root formation was regulated by auxin, cytokinin, ethylene and brassinosteroid related genes, and transcribed with GRAS, AP2, ERF, LBD, SRS and WRKY families Factor expression is closely related. It can be seen from the above that adventitious roots of pepper hypocotyls are involved in a series of extremely complicated organogenesis processes.