作者用免疫吸印(IB)法系统地研究了感染HCMV孕妇和急性肝炎病人对HCMV结构蛋白的免疫应答。用人胚成纤维细胞培养HCMV(Towne株),增殖后进行纯化。用SDS-PAGE法分离多肽,然后电转移至硝酸纤维素膜上,将膜切成4～5mm的条,用3%明胶Tris缓冲盐水(TBS)处理1小时,再用1:50稀释的人血清温育过夜。用TBS The authors systematically investigated the immune response to HCMV structural proteins in HCMV pregnant women and acute hepatitis patients using immunoblotting (IB). HCMV (Towne strain) was cultured with human embryonic fibroblasts and propagated for purification. Polypeptides were separated by SDS-PAGE and electrotransferred to nitrocellulose membranes. The membranes were cut into 4-5 mm strips, treated with 3% gelatin Tris-buffered saline (TBS) for 1 hour and then diluted 1:50 Serum was incubated overnight. With TBS