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目的检测脆性组氨酸三联体(FHIT)基因在胃癌中的蛋白表达及其甲基化情况,探讨FHIT在胃癌中的作用。方法收集胃癌新鲜组织标本共33例,采用免疫组织化学和甲基化特异性聚合酶链反应(PCR)的方法检测FHIT基因的蛋白表达和异常甲基化。结果 33例胃癌组织中,24例表达FHIT蛋白,阳性率为73％,有13例发生了甲基化,阳性率为39％。胃癌中FHIT基因的蛋白表达和异常甲基化呈负相关。结论 FHIT基因的异常甲基化是引起FHIT基因失活并导致胃癌发生的重要机制。
Objective To investigate the protein expression and methylation status of FHIT gene in gastric cancer and to explore the role of FHIT in gastric cancer. Methods Totally 33 fresh gastric cancer specimens were collected. The protein expression and abnormal methylation of FHIT gene were detected by immunohistochemistry and methylation specific polymerase chain reaction (PCR). Results Of the 33 cases of gastric carcinoma, 24 cases expressed FHIT protein, the positive rate was 73%. Thirteen cases had methylation and the positive rate was 39%. FHIT gene protein expression and abnormal methylation were negatively correlated in gastric cancer. Conclusion Abnormal methylation of FHIT gene is an important mechanism of inactivation of FHIT gene and its occurrence in gastric cancer.