一般离子化物质,如:胺类及有机酸类的液相层析在层析分离技术中是一个重要的课题,存在着保留值、柱效及峰对称等一系列问题。如果采用吸附层析法来进行分离,则需要应用极性洗脱剂,但极性洗脱剂又常使吸附表面的均一性受到破坏而导致严重的“拖尾”。有时应用酸性或碱性缓冲液作为洗脱剂 General ionization substances, such as: liquid chromatography of amines and organic acids in the chromatographic separation technology is an important issue, there are a series of issues such as retention, efficiency and peak symmetry. Polar eluents need to be applied if they are separated by adsorption chromatography, but polar eluents, in turn, often disrupt the homogeneity of the adsorption surface, resulting in severe “tailing.” Acidic or alkaline buffers are sometimes used as eluents