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以阿月浑子与黄连木的杂种果实和杂种幼胚为试材,对果实生长发育规律及幼胚培养进行了研究,探讨了胚龄、培养基类型和6-BA浓度等对杂种胚培苗再生的影响。结果表明:(1)杂种果实的纵、横径生长呈“S”型曲线变化,果实鲜质量生长呈双“S”型曲线变化。(2)杂种胚的发育始于授粉后80d,80~100d是杂种胚败育的关键时期。(3)授粉后80d的杂种幼胚难以成苗,授粉后100和120d的杂种幼胚成苗率分别为37.04%和83.33%。(4)适宜杂种胚培苗增殖的培养基为:DKW+2.0mg·L-16-BA+0.05g·L-1IBA+0.3g·L-1LH+1.5g·L-1PVP,增殖系数为3.47,苗高为3.32cm。(5)适宜杂种胚培苗生根的培养基为:1/2WPM+2.0mg·L-1IBA+0.05mg·L-1NAA,生根率为80%。
Taking the hybrid fruits and hybrid embryos of Pistachio and Pistacia chinensis as test materials, the law of fruit growth and development and immature embryo culture were studied. The effects of embryo age, medium type and 6-BA concentration The impact of seedling regeneration. The results showed that: (1) The vertical and horizontal diameter growth of hybrid fruits showed a curve of “S” shape, and the change of fresh “S” shape of fruits showed a double “S” pattern. (2) The development of hybrid embryos began 80 days after pollination, and 80-100 days was the critical period for hybrid abortion. (3) Hybrid embryos 80 days after pollination were difficult to mature, and the seedling rates of hybrid embryos 100 and 120 days after pollination were 37.04% and 83.33% respectively. (4) The medium suitable for the proliferation of hybrid embryo culture was: DKW + 2.0mg · L-16-BA + 0.05g · L-1IBA + 0.3g · L-1LH + 1.5g · L-1PVP with multiplication coefficient of 3.47 , Seedling height is 3.32cm. (5) The suitable culture medium for hybrid embryo culture was 1 / 2WPM + 2.0mg · L-1IBA + 0.05mg · L-1NAA, and the rooting rate was 80%.