论文部分内容阅读
目的观察鹿茸多肽对大鼠关节软骨细胞体外传代培养中出现的去分化现象的作用。方法将第3代软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组传代,使之进入第4代,同时以第2代软骨细胞为对照组,进行阿力新蓝染色法检测GAG(glycosaminoglycan)含量和结构,RT-PCR(reverese transcript-polymerase chain reaction)法检测Ⅱ型胶原、Aggrecan蛋白,对鹿茸多肽抗软骨细胞去分化进行分子生物学研究。结果鹿茸多肽对软骨细胞胞外基质中GAG、Ⅱ型胶原和Aggrecan蛋白表达有显著促进作用(P<0.01),其作用优于硫酸氨基葡萄糖。结论鹿茸多肽对体外培养的软骨细胞去分化现象有抑制作用,其作用优于硫酸氨基葡萄糖。
Objective To observe the effect of pilose antler polypeptide on dedifferentiation of rat articular chondrocytes in vitro. Methods The third generation chondrocytes were divided into blank control group, different concentrations of pilose antler polypeptide group, and different concentrations of glucosamine sulfate group were passaged into the fourth generation. At the same time, second generation chondrocytes were used as the control group to perform alixin blue. The content and structure of GAG (glycosaminoglycan) were detected by staining, and type II collagen and Aggrecan protein were detected by RT-PCR (reverese transcript-polymerase chain reaction) method. The anti-differentiation of pilose antler polypeptide against chondrocyte was studied by molecular biology. Results The velvet antler polypeptide had a significant effect on the expression of GAG, type II collagen and Aggrecan protein in extracellular matrix of chondrocytes (P<0.01), and its effect was better than that of glucosamine sulfate. Conclusion Pilose Antler polypeptide can inhibit the dedifferentiation of cultured chondrocytes in vitro, and its effect is better than that of glucosamine sulfate.