取 Wistar 大鼠生后1～3天乳鼠的心室肌组织,用0.06%的胰蛋白酶制备细胞悬液,在含15%小牛血清的培养液中培养48小时后用于实验。在倒置显微镜的保温罩内观察心肌细胞团的搏动。用一个自制装置,将因细胞搏动产生的光强度变化转换成电讯号,经放大后记录于多导生理仪上。 Wistar rats were sacrificed 1 to 3 days after birth, the neonatal rat ventricular muscle tissue with 0.06% trypsin cell suspension preparation, cultured in a medium containing 15% fetal calf serum 48 hours after the experiment. Inspect the pulsation of the cardiomyocyte mass in a thermostated cover of an inverted microscope. Using a self-made device, the change in light intensity due to the pulsation of the cells is converted into an electrical signal which is amplified and recorded on a multi-guide physiology instrument.