这里所叙述的是一种新的简单、灵敏、快速对氯化汞进行定性及定量测定的纸层析—酶催化法和微量薄层层析—酶催化法。该法是通过琥珀酸盐脱氢酶抑制反应检定氯化汞,2—(4—碘苯)—3—(4—硝基苯)5—苯四唑啉氯化物和N—甲基苯唑啉硫酸甲酯作显色剂,可以测定0.5～5μg的氯化汞,可检至50μg的氯化汞。该法能用于未经净化的淡水及海水中氯化汞的定量测定和硫酸铜的检定。运用薄层层析—酶催化法,通过胆碱酯酶抑制反应可对有机磷化合物进行μg水平的检定和测定。通常,酶催化法较化学法灵敏、具有特异性。而应用纸层析和微量薄层层析检定、测定重金属化合物(如危险的环境污染物—氯化汞)未有报道过。这是首次报道以琥珀酸盐 Described here is a new simple, sensitive and rapid method for the qualitative and quantitative determination of mercuric chloride by paper chromatography-enzyme catalysis and micro-thin-layer chromatography-enzyme catalysis. This method is based on the determination of mercuric chloride, 2- (4-iodobenzene) -3- (4-nitrophenyl) -5-phenyltetrazolinium chloride and N-methyloxazole by succinate dehydrogenase inhibition reaction Methyl methionine as a color reagent, can measure 0.5 ~ 5μg of mercury chloride, can detect 50μg of mercury chloride. The method can be used for the quantitative determination of mercuric chloride in fresh and seawater without purification and the determination of copper sulfate. Using thin-layer chromatography-enzyme catalysis method, the level of μg of organophosphorus compounds can be tested and determined by cholinesterase inhibition reaction. In general, enzyme-catalyzed method is more sensitive and specific than chemical method. However, the determination of heavy metal compounds (such as hazardous environmental pollutants - mercuric chloride) by paper chromatography and micro-TLC has not been reported. This is the first time that succinate has been reported